The main unit of the 11,184-acre refuge is located about 7 miles north of the city of Eufaula, Alabama, along both banks of the Chattahoochee River in southeast Alabama and southwest Georgia. Wetlands, croplands, woodlands, old fields, grasslands, and open water create a mosaic of wildlife-rich habitats that support almost 300 species of birds, 40 species of mammals, and many species of amphibians, reptiles and fishes.
Located in the southeast part of the state, Clayton has been the county seat of Barbour County since 1834. Though largely untouched during the Civil War, the town was visited by a Union raiding party in April 1865 after the war had ended.
Barbour County is a county Alabama. As of the 2010 census, the population was 27,457. Its county seat is Clayton Alabama. Its name is in honor of James Barbour, who served as Governor of Virginia.