The main unit of the 11,184-acre refuge is located about 7 miles north of the city of Eufaula, Alabama, along both banks of the Chattahoochee River in southeast Alabama and southwest Georgia. Wetlands, croplands, woodlands, old fields, grasslands, and open water create a mosaic of wildlife-rich habitats that support almost 300 species of birds, 40 species of mammals, and many species of amphibians, reptiles and fishes.